Enzymes and Inhibitors
Bioworld Trypsin, TPCK-Treated, 50 mg
Protease which cleaves specifically at the -COOH side of arginie and lysine. TPCK-treated Trypsin is a serine endopeptidate. Trypsin is treated with TPCK to inactivate any remaining chymotryptic activity. This type of trypsin is modified by acetyltion of the sigma-amino groups of lysine residues to prevent autolysis.
Bioworld Trypsin, TPCK-Treated, 100 mg
Protease which cleaves specifically at the -COOH side of arginie and lysine. TPCK-treated Trypsin is a serine endopeptidate. Trypsin is treated with TPCK to inactivate any remaining chymotryptic activity. This type of trypsin is modified by acetyltion of the sigma-amino groups of lysine residues to prevent autolysis.
Bioworld alpha-Chymotrypsin, 500 mg
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
Bioworld alpha-Chymotrypsin, 1 g
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
Bioworld IPTG, 25 g
IPTG Isopropyl-β-D-Thiogalactopyranoside. An inducer of β-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1mM final IPTG concentration in LB (Luria Broth) media is recommended. Techncial Information:Biotechnology Grade.Dioxane free.
Bioworld Beta-Glactosidase, 1000 Unit(s)
Beta-Galactosidase Enzyme found in lac+ bacterial cells that catalyzes the cleavage of -linked terminal galactose-containing substratesLyophilized powderSource: E. coliConjugation gradeActivity (37°C):800-1000U/mg proteinUnit definition: One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH7.3 at 37°C, 410nm.Aggregation (HPLC): <3.0%Application: Used as an indicator enzyme conjugated to proteins such as antibodies.
Bioworld DNase I (Bovine Pancreas), 50 KU
DNase I DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3'-OH oligonucleotides. Mg+2 in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence on Mn+2, DNase I cleaves both strands of the DNA. DNase I is useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. It is also used in purifying RNA from cells or transcription mixtures. Specifications:CAS: 9003-98-9EC: 232-667-0Source: Bovine pancreasActivity: ≥10000 U/mg.Ultra Pure GradeOur DNase I is guaranteed free of RNase contaminants.
Bioworld BCIP, 1 g
BCIP Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue, insoluble product. This product then reacts to form a chromogen homodimer. This reaction may be used to amplify the signal by catalyzing the reduction of a tetrazolium salt (usually NBT) to form an intenselycolored, insoluble formazan chromogen. Technical Information:Ultra Pure GradePurity (enzymatic): >99.0%Purity (UV): >99.0%Inhibitors (enzymatic): <0.1%Solubility (0.1% phosphate buffer): Clear and haze-freeSolubility (0.10% DMF): Pass (Clear/Haze-free)Melting Point: 195-197CA260 (1mg/ml, DMF): 0.010Ref: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
![Bioworld Cellulase RS [Onozuka RS], 5 g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50198965.jpg-250.jpg)
Bioworld Cellulase RS [Onozuka RS], 5 g
From mutant Trichoderma viride derived from the parent strain for Cellulase Onozuka R-10. Cellulase RS contains a high activity of decomposing natural celluloses. Can be used to obtain protoplasts and dissolve cell walls in a wide range of plants.* Soluble in water* Optimum pH range 4.0-5.0* Optimum temperature range 50° to 60°CUsually ships within 24 hours.This product is sold for laboratory research use only.
Bioworld Malate Dehydrogenase, 2.5 KU
Malate DehydrogenaseSpecification:Malate Dehydrogenase, Ammonium Sulfate SuspensionUltra Pure GradeSource: Porcine heartSpecific activity (25 °C): > 1200 U/mgConcentration: > 12,000 U/mLAlanine aminotransferase: < 0.005%Aspartate aminotransferase: < 0.005%Lactate dehydrogenase: < 0.005%Unit Definition: The amount of enzyme which will convert 1 micromole of oxaloacetate and NADH per minute at 25 C in phosphate buffer at pH 7.5.
Bioworld IPTG (Isopropyl-B-D-ThioGalactopyranoside) Solution, 100mM, 1 mL
Isopropyl-β-D-Thiogalactopyranoside, an inducer of β-galactosidase is used to promote the expression of proteins in cells controlled by the lac operator system. Supplied as 1ml or 10x1ml of 100mM ready-to-use solution to be used with X-gal for blue white colony screening. Techncial Information:Biotechnology Grade.Ready-to-use solution Sterile filtered (0.22μM)Dioxane freeApplication: Petriplates: Use 40 μl per Petriplates (9 cm diameter). LB agar: Use 1μl/mL of liquid LB agar medium just before pouring (~50°C).Liquid culture: Use 1μl/mL of liquid culture medium.Sold as 1mL or 10 x 1mL.
Bioworld Macerozyme R-10, 5 g
Macerozyme R-10Macerating enzyme from Rhizopus sp. suitable for the isolation of plant cells and can be used in combination with cellulase.Specification:Used in plant protoplast preparation to digest cell wall prior to oranelle isolation.A multi-component enzyme mixture containing approximately: * Cellulase: 0.1 U/mg * Hemicellulase: 0.25 U/mg * Pectinase: 0.5 U/mgSource: Rhizopus sp.Solubility: Soluble in water
Bioworld Cellulase (Onozuka R-10), 5 g
Cellulase (Onozuka R-10) From Trichadema Viride. Enzyme mixture often used in combination with Macerozyme R-10 (M22010) for isolation of plant protoplasts. One unit of Cellulase will liberate 1.0 µmole of glucose from carboxymethyl cellulose.Optimum pH range: 4-5.Soluble in water (10mg/ml)Contents:Cellulase: 1.0 U/mg Pectinase: 0.4 U/mg Hemicellulase: 1.0 U/mg alpha-Amylase: 0.6 U/mg Protease: 0.01 U/mg
Bioworld X-Gal-IPTG, Ready-to-Use Spray, 20 mL
X-Gal-IPTG, Ready- to-use, SprayStable X-gal/IPTG non-toxic solution For use in blue/white colony screening in expression studiesDoes not contain toxic DMF and DMSOContains 40 mg/ml of IPTG (Isopropyl-β-D-Thiogalactopyranoside) and 40 mg/ml of X-gal (5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside)Supplied in a very easy to use and convenient to use Spray BottleAlso available in a Dropper Bottle (cat# 21530077-1)Use: Spray 2-3 times on desired bacterial plate Spread and ensure absorption (wait ~5-10 minutes) Plate the transformed bacteria to be tested and incubate
Bioworld Macerozyme R-10, 1 g
Macerozyme R-10Macerating enzyme from Rhizopus sp. suitable for the isolation of plant cells and can be used in combination with cellulase.Specification:Used in plant protoplast preparation to digest cell wall prior to oranelle isolation.A multi-component enzyme mixture containing approximately: * Cellulase: 0.1 U/mg * Hemicellulase: 0.25 U/mg * Pectinase: 0.5 U/mgSource: Rhizopus sp.Solubility: Soluble in water